Hepatoprotective activity of the ethanolic extract of Morus indica roots from Indian Bodo tribes

SN Applied Sciences

Talambedu Usha¹  Arvind Kumar Goyal²  Hankhray Boro²  Sandeep Das²  Hemavathy Ekambaram³  Prakashmurthy Chandana³  Sushil Kumar Middha³  Hasan Soliman Yusufoglu⁴  Dinesh Babu⁵ 

[1] Department of Biochemistry, Bangalore University;Department of Biotechnology, Bodoland University;Department of Biotechnology, Maharani Lakshmi Ammanni College for Women;Department of Pharmacognosy and Pharmaceutical Chemistry, College of Dentistry and Pharmacy, Buraydah Private Colleges;Katz Group-Rexall Centre for Pharmacy and Health Research, University of Alberta;

关键词: Morus indica L.;    Mulberry;    Polyphenols;    Hepatoprotective;    Glutathione metabolism;   

DOI  :  10.1007/s42452-021-04859-z

来源: DOAJ

【 摘 要 】

Abstract The roots of Morus species are well described in the Pharmacopoeia of the People's Republic of China (ChP) for its traditional use in treating liver fibrosis due to its hepatoprotective property. However, little is known about the hepatoprotective effect of the roots of Morus indica L. (RoMi), and the pharmacological mechanism(s) are uncertain due to its intricacy. Therefore, this study evaluates the hepatoprotective activity of the ethanolic extract of RoMi (eRoMi) against the CCl4-induced in-vivo animal model at different dosages (100 and 200 mg/kg BW) in comparison with silymarin as a positive control. The hepatoprotective activity of eRoMi was evaluated by measuring the levels of serum biomarkers, hepatic antioxidant enzymes and was verified by histological studies. Interestingly, 1,2-bis(trimethylsilyl) benzene, 1,4-phenylenebis (trimethylsilane), 2,4,6-cycloheptatriene-1-one, 3,5-bis-trimethylsilyl and α-amyrin were the active components found in eRoMi as detected by GC–MS. Oral administration of eRoMi (200 mg/kg BW) to rats significantly protected serum biochemical parameters (increased ALT, AST, LDH, bilirubin and GGT as well as depletion of antioxidant enzymes and hepatic GSH) and elevation in hepatic lipid peroxidation as compared to CCl4-treated rats. The hematological indices such as erythrocytes, hemoglobin, monocytes and lymphocytes were also normal in eRoMi-treated rats. The histopathological evaluation indicated a significant restoration of liver structure as compared to silymarin. This study is the first scientific validation for the traditional use of eRoMi to understand its hepatoprotective activity.

【 授权许可】

Unknown