An Innovative Customized Biomimetic Hydrogel for Drug Screening Application Potential: Biocompatibility and Cell Invasion Ability

International Journal of Molecular Sciences

Bai-Hung Huang¹  Yung-Chieh Cho¹  Hsu-An Pan¹  Keng-Liang Ou²  Wen-Chien Lan³  Hsin-Yu Tsai⁴  Takashi Saito⁴  Kuo-Sheng Hung⁵  Chiung-Fang Huang⁶  Yung-Kang Shen⁶  Hsin-Hua Chou⁷ 

[1] Biomedical Technology R&D Center, China Medical University, Taichung 404, Taiwan;Department of Dentistry, Taipei Medical University-Shuang Ho Hospital, New Taipei City 235, Taiwan;Department of Oral Hygiene Care, Ching Kuo Institute of Management and Health, Keelung 203, Taiwan;Division of Medicine for Function and Morphology of Sensor Organs, Department of Dentistry and Oral Surgery, Osaka Medical College, Osaka 569-8686, Japan;Graduate Institute of Injury Prevention and Control, College of Public Health, Taipei Medical University, Taipei 110, Taiwan;School of Dental Technology, College of Oral Medicine, Taipei Medical University, Taipei 110, Taiwan;School of Oral Hygiene, College of Oral Medicine, Taipei Medical University, Taipei 110, Taiwan;

关键词: Pluronic F127;    RGD peptide;    spheroid;    hydrogel;    drug screening;   

DOI  :  10.3390/ijms23031488

来源: DOAJ

【 摘 要 】

The ability of Pluronic F127 (PF127) conjugated with tetrapeptide Gly-Arg-Gly-Asp (GRGD) as a sequence of Arg-Gly-Asp (RGD) peptide to form the investigated potential hydrogel (hereafter referred to as 3DG bioformer (3BE)) to produce spheroid, biocompatibility, and cell invasion ability, was assessed in this study. The fibroblast cell line (NIH 3T3), osteoblast cell line (MG-63), and human breast cancer cell line (MCF-7) were cultured in the 3BE hydrogel and commercial product (Matrigel) for comparison. The morphology of spheroid formation was evaluated via optical microscopy. The cell viability was observed through cell counting Kit-8 assay, and cell invasion was investigated via Boyden chamber assay. Analytical results indicated that 3BE exhibited lower spheroid formation than Matrigel. However, the 3BE appeared biocompatible to NIH 3T3, MG-63, and MCF-7 cells. Moreover, cell invasion ability and cell survival rate after invasion through the 3BE was displayed to be comparable to Matrigel. Thus, these findings demonstrate that the 3BE hydrogel has a great potential as an alternative to a three-dimensional cell culture for drug screening applications.

【 授权许可】

Unknown