Protocol for assessing ex vivo lipolysis of murine adipose tissue

STAR Protocols

Julia M. Myers¹  Andrea Tedeschi²  Debasish Roy² 

[1]Corresponding author

[2]Department of Neuroscience, Wexner Medical Center, The Ohio State University, Columbus, OH 43210, USA

关键词: Cell-based Assays;    Metabolism;   

DOI  :  

来源: DOAJ

【 摘 要 】

Summary: Here, we provide a detailed protocol for assessing ex vivo lipolysis of subcutaneous and visceral white adipose tissue. We describe a robust approach to detect depot-specific changes in lipolytic potential under basal and beta-adrenergic receptor-stimulated conditions. Given that adipose tissue plays a critical role in systemic metabolic health, this experimental protocol can be used to determine changes in adipose tissue function in health and disease. : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.

【 授权许可】

Unknown