Protocol to record and quantify the intracellular pH in contracting cardiomyocytes

STAR Protocols

Xiao-Dong Zhang¹  Valeriy Timofeyev²  Yankun Lyu²  Nipavan Chiamvimonvat²  James Overton²  Phung N. Thai²  Ebenezer N. Yamoah³ 

[1] Department of Veterans Affairs, Northern California Health Care System, Mather, CA 95655, USA;Department of Internal Medicine, University of California, Davis, Davis, CA 95616, USA;Department of Physiology and Cell Biology, University of Nevada, Reno School of Medicine, Reno, NV 89557, USA;

关键词: Cell isolation;    Single Cell;    Cell-based Assays;    Metabolism;    Microscopy;   

DOI  :  

来源: DOAJ

【 摘 要 】

Summary: Intracellular pH (pHi) plays critical roles in the regulation of cardiac function. Methods and techniques for cardiac pHi measurement have continued to evolve since early 1960s. Fluorescent microscopy is the most recently developed technique with several advantages over other techniques including higher spatial and temporal resolutions, and feasibility for contracting cell measurement. Here, we describe detailed methods for mouse cardiomyocyte isolation, and simultaneous measurement and quantification of pHi and sarcomere length in contracting cardiomyocytes.For complete details on the use and execution of this protocol, please refer to Lyu et al. (2022).

【 授权许可】

Unknown