AANAT1 functions in astrocytes to regulate sleep homeostasis

eLife

Gregory Artiushin¹  Donald J van Meyel²  Yongjun Li²  Sally Li³  Amita Sehgal⁴  Sejal Davla⁴  Daryan Chitsaz⁵ 

[1] BRaIN Program, Research Institute of the McGill University Health Centre, Montreal, Canada;Integrated Program in Neuroscience, McGill University, Montreal, Canada;Biology Graduate Group, University of Pennsylvania, Philadelphia, United States;Centre for Research in Neuroscience, Department of Neurology and Neurosurgery, McGill University, Montreal, Canada;Neuroscience Graduate Group, Perelman School of Medicine, University of Pennsylvania, Philadelphia, United States;

关键词: astrocytes;    AANAT1;    monoamines;    sleep;    dopamine;    serotonin;   

DOI  :  10.7554/eLife.53994

来源: DOAJ

【 摘 要 】

How the brain controls the need and acquisition of recovery sleep after prolonged wakefulness is an important issue in sleep research. The monoamines serotonin and dopamine are key regulators of sleep in mammals and in Drosophila. We found that the enzyme arylalkylamine N-acetyltransferase 1 (AANAT1) is expressed by Drosophila astrocytes and specific subsets of neurons in the adult brain. AANAT1 acetylates monoamines and inactivates them, and we found that AANAT1 limited the accumulation of serotonin and dopamine in the brain upon sleep deprivation (SD). Loss of AANAT1 from astrocytes, but not from neurons, caused flies to increase their daytime recovery sleep following overnight SD. Together, these findings demonstrate a crucial role for AANAT1 and astrocytes in the regulation of monoamine bioavailability and homeostatic sleep.

【 授权许可】

Unknown