Tracking distinct microglia subpopulations with photoconvertible Dendra2 in vivo

Journal of Neuroinflammation

Kaitryn E. Ronning¹  Marie E. Burns¹  Eric B. Miller¹  Sarah J. Karlen² 

[1] Center for Neuroscience, University of California;Department of Cell Biology and Human Anatomy, University of California;

关键词: Retina;    Photoreceptor;    Microglia;    Fluorescent protein;    Imaging;   

DOI  :  10.1186/s12974-021-02285-x

来源: DOAJ

【 摘 要 】

Key points New mouse for tracking microglia and all mononuclear phagocytes both ex and in vivo within the CNS over time. Dendra2 protein is stable enough to survive tissue processing for immunohistochemistry and flow cytometry quantification. Simultaneous fluorescence imaging of Dendra2 and light scattering measurements can be used to assess the immune response to retinal damage. Chronic in vivo imaging reveals mixed populations of microglia and monocytes during retinal degeneration.

【 授权许可】

Unknown