Multi-color localization microscopy of fixed cells as a promising tool to study organization of bacterial cytoskeleton

2nd International School and Conference Saint-Petersburg OPEN on Optoelectronics, Photonics, Engineering and Nanostructures

Vedyaykin, A.D.^1 ; Gorbunov, V.V.^1 ; Sabantsev, A.V.^1 ; Polinovskaya, V.S.^1 ; Vishnyakov, I.E.^2 ; Melnikov, A.S.^1 ; Serdobintsev, P.Yu.^1 ; Khodorkovskii, M.A.^1

Research Institute of Nanobiotechnologies, Peter the Great St.Petersburg Polytechnic University, Polytechnicheskaya 29, Saint-Petersburg, Russia^1

Institute of Cytology, Russian Academy of Sciences, Tikhoretsky av. 4, Saint Petersburg

194064, Russia^2

关键词: Biological structures;    Different structure;    Diffraction limited;    Diffraction limits;    Fluorescent protein;    Spatial arrangements;    Temperature sensitive;    Visualization method;   

Others  :  https://iopscience.iop.org/article/10.1088/1742-6596/643/1/012020/pdf
DOI  :  10.1088/1742-6596/643/1/012020

来源: IOP

【 摘 要 】

Localization microscopy allows visualization of biological structures with resolution well below the diffraction limit. Localization microscopy was used to study FtsZ organization in Escherichia coli previously in combination with fluorescent protein labeling, but the fact that fluorescent chimeric protein was unable to rescue temperature-sensitive ftsZ mutants suggests that obtained images may not represent native FtsZ structures faithfully. Indirect immunolabeling of FtsZ not only overcomes this problem, but also allows the use of the powerful visualization methods arsenal available for different structures in fixed cells. In this work we simultaneously obtained super-resolution images of FtsZ structures and diffraction-limited or super-resolution images of DNA and cell surface in E. coli, which allows for the study of the spatial arrangement of FtsZ structures with respect to the nucleoid positions and septum formation.