Daily Exposure to a Cranberry Polyphenol Oral Rinse Alters the Oral Microbiome but Not Taste Perception in PROP Taster Status Classified Individuals

Nutrients

Yan Y. Lam¹  Liping Zhao¹  Guojun Wu¹  Melania Melis²  Iole Tomassini Barbarossa²  Mariano Mastinu²  Neeta Y. Yousaf³  Beverly J. Tepper³  Cristina Contini⁴  Tiziana Cabras⁴ 

[1] Department of Biochemistry and Microbiology, Center for Microbiome, Nutrition, and Health, New Jersey Institute for Food, Nutrition, and Health, Rutgers University, New Brunswick, NJ 08901, USA;Department of Biomedical Sciences, University of Cagliari, 09042 Monserrato, Italy;Department of Food Science & Center for Sensory Sciences & Innovation, Rutgers University, New Brunswick, NJ 08901, USA;Department of Life and Environmental Sciences, University of Cagliari, 09042 Monserrato, Italy;

关键词: cranberry polyphenols;    oral microbiota;    salivary proteins;    6-n-propylthiouracil;    TAS2R38;    PROP phenotype;   

DOI  :  10.3390/nu14071492

来源: DOAJ

【 摘 要 】

Diet and salivary proteins influence the composition of the oral microbiome, and recent data suggest that TAS2R38 bitter taste genetics may also play a role. We investigated the effects of daily exposure to a cranberry polyphenol oral rinse on taste perception, salivary proteins, and oral microbiota. 6-n-Propylthiouracil (PROP) super-tasters (ST, n = 10) and non-tasters (NT, n = 10) rinsed with 30 mL of 0.75 g/L cranberry polyphenol extract (CPE) in spring water, twice daily for 11 days while consuming their habitual diets. The 16S rRNA gene sequencing showed that the NT oral microbiome composition was different than that of STs at baseline (p = 0.012) but not after the intervention (p = 0.525). Principal coordinates analysis using unweighted UniFrac distance showed that CPE modified microbiome composition in NTs (p = 0.023) but not in STs (p = 0.096). The intervention also altered specific salivary protein levels (α-amylase, MUC-5B, and selected S-type Cystatins) with no changes in sensory perception. Correlation networks between oral microbiota, salivary proteins, and sensory ratings showed that the ST microbiome had a more complex relationship with salivary proteins, particularly proline-rich proteins, than that in NTs. These findings show that CPE modulated the oral microbiome of NTs to be similar to that of STs, which could have implications for oral health.

【 授权许可】

Unknown