Comparison of Throat Washings, Nasopharyngeal Swabs and Oropharyngeal Swabs for Detection of SARS-CoV-2

Viruses

Florian Hitzenbichler¹  Bernd Salzberger¹  Stilla Bauernfeind¹  JürgenJ. Wenzel²  Barbara Schmidt³ 

[1] Department of Infection Prevention and Infectious Diseases, University Medical Center Regensburg, 93053 Regensburg, Germany;Institute of Clinical Microbiology and Hygiene, University Medical Center Regensburg, 93053 Regensburg, Germany;Institute of Medical Microbiology and Hygiene, University of Regensburg, 93053 Regensburg, Germany;

关键词: SARS-CoV-2;    COVID-19;    throat washing;    nasopharyngeal swab;    oropharyngeal swab;    saliva;   

DOI  :  10.3390/v13040653

来源: DOAJ

【 摘 要 】

Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) RNA is detected by reverse-transcription quantitative real-time PCR (RT-qPCR) from respiratory specimens. This study compares throat washings (TW), nasopharyngeal swabs (NS) and oropharyngeal swabs (OS). A total of 102 samples from 34 adult patients with confirmed SARS-CoV-2 infection were analysed by RT-qPCR with absolute quantification. The median concentrations and diagnostic sensitivities were $5.8\times {10}^4$ copies/mL, 85% (NS), $1.4\times {10}^4$, 79% (OS) and $4.3\times {10}^3$, 85% (TW). Concentration differences were significant between NS and TW (P = 0.019). Saliva (SA) was available from 21 patients (median $3.4\times {10}^3$). OS and TW can be considered for SARS-CoV-2 diagnostics, although with slightly lower concentrations.

【 授权许可】

Unknown