Investigating the Viability of Epithelial Cells on Polymer Based Thin-Films

Polymers

Boštjan Vihar¹  Marko Milojević¹  Laura Činč Ćurić¹  Uroš Maver¹  Jan Rožanc¹  Boštjan Krajnc¹  Lidija Gradišnik¹ 

[1] Faculty of Medicine, Institute of Biomedical Sciences, University of Maribor, Taborska Ulica 8, SI-2000 Maribor, Slovenia;

关键词: polymers;    thin films;    viability;    morphology;    HUVEC;    HUIEC;   

DOI  :  10.3390/polym13142311

来源: DOAJ

【 摘 要 】

The development of novel polymer-based materials opens up possibilities for several novel applications, such as advanced wound dressings, bioinks for 3D biofabrication, drug delivery systems, etc. The aim of this study was to evaluate the viability of vascular and intestinal epithelial cells on different polymers as a selection procedure for more advanced cell-polymer applications. In addition, possible correlations between increased cell viability and material properties were investigated. Twelve polymers were selected, and thin films were prepared by dissolution and spin coating on silicon wafers. The prepared thin films were structurally characterized by Fourier transform infrared spectroscopy, atomic force microscopy, and goniometry. Their biocompatibility was determined using two epithelial cell lines (human umbilical vein endothelial cells and human intestinal epithelial cells), assessing the metabolic activity, cell density, and morphology. The tested cell lines showed different preferences regarding the culture substrate. No clear correlation was found between viability and individual substrate characteristics, suggesting that complex synergistic effects may play an important role in substrate design. These results show that a systematic approach is required to compare the biocompatibility of simple cell culture substrates as well as more complex applications (e.g., bioinks).

【 授权许可】

Unknown