Expression of Aspergillus oryzae Tannase in Pichia pastoris and Its Application in the Synthesis of Propyl Gallate in Organic Solvent

Food Technology and Biotechnology

Xiao-Wei Yu¹  Yong-Quan Li² 

[1] Jiangnan University College of Biotechnology, CN-214122 Wuxi, PR China;Zheijang University College of Life Science, CN-310058 Hangzhou, PR China;

关键词: tannase;    Pichia pastoris;    recombinant protein production;    propyl gallate;    biosynthesis;   

DOI  :  

来源: DOAJ

【 摘 要 】

Gallic acid esters could be synthesized biologically by tannase in organic media, among which is propyl gallate, an antioxidant widely used as a food additive. Efficient intracellular expression of Aspergillus oryzae tannase was achieved in Pichia pastoris under the control of the AOX1 promoter, and the productivity of recombinant tannase was 960 U/L or 64 U/g dry mass. The recombinant P. pastoris was used to synthesize propyl gallate in organic solvent and the yield of propyl gallate was 53 %.

【 授权许可】

Unknown