期刊论文详细信息
Food Technology and Biotechnology | |
Expression of Aspergillus oryzae Tannase in Pichia pastoris and Its Application in the Synthesis of Propyl Gallate in Organic Solvent | |
Xiao-Wei Yu1  Yong-Quan Li2  | |
[1] Jiangnan University College of Biotechnology, CN-214122 Wuxi, PR China;Zheijang University College of Life Science, CN-310058 Hangzhou, PR China; | |
关键词: tannase; Pichia pastoris; recombinant protein production; propyl gallate; biosynthesis; | |
DOI : | |
来源: DOAJ |
【 摘 要 】
Gallic acid esters could be synthesized biologically by tannase in organic media, among which is propyl gallate, an antioxidant widely used as a food additive. Efficient intracellular expression of Aspergillus oryzae tannase was achieved in Pichia pastoris under the control of the AOX1 promoter, and the productivity of recombinant tannase was 960 U/L or 64 U/g dry mass. The recombinant P. pastoris was used to synthesize propyl gallate in organic solvent and the yield of propyl gallate was 53 %.
【 授权许可】
Unknown