Near-Normalized Gene Expression Profiles in Bladder With Detrusor Overactivity in Rats With Bladder Outlet Obstruction After Deobstruction

International Neurourology Journal

Hyunzu Kim¹  Chang-Shin Park²  Bo-Hwa Choi²  Ju-Hee Kang²  Tack Lee³  U-Sung Lim³  Sang-Min Yoon³  Dong Hyuk Kang³  Hae-Do Jung⁴ 

[1] Department of Anesthesiology and Pain Medicine, Inha University College of Medicine, Incheon, Korea;Department of Pharmacology, Hypoxia-Related Disease Research Center, Inha Research Institute for Medical Sciences, Inha University College of Medicine, Incheon, Korea;Department of Urology, Inha University College of Medicine, Incheon, Korea;Department of Urology, Yongin Severance Hospital, Yonsei University College of Medicine, Yongin, Korea;

关键词: Urethral Obstruction;    Urinary Bladder, Overactive;    DNA;    Microarray Analysis;    Gene Expression Profiling;   

DOI  :  10.5213/inj.1732774.387

来源: DOAJ

【 摘 要 】

Purpose The pathophysiological role of detrusor overactivity (DO) in the bladder, which is commonly observed in various bladder diseases, is not well understood. DO appears in bladder outlet obstruction (BOO), and may continue even after subsequent deobstruction. DO therefore provides an excellent opportunity to observe molecular biological changes. Methods In this study, to understand the molecular effects of persistent DO after BOO induction and deobstruction, we performed awake cystometry on female Sprague-Dawley rats divided into 4 groups: a sham group, a BOO group, a deobstructed group with DO after BOO (DDO), and a deobstructed group without DO after BOO (non-DDO). Total RNA was extracted from the bladder samples, and gene expression profiles were compared between the sham and model groups. Results DO was observed in 5 of the 6 rats (83%) in the BOO group, and in 6 of the 13 rats (46%) in the deobstructed group. The non-DDO group showed a significantly greater residual volume than the DDO group. Through a clustering analysis of gene expression profiles, we identified 7,532 common upregulated and downregulated genes, the expression of which changed by more than 2 fold. In the BOO group, 898 upregulated and 2,911 downregulated genes were identified. The non-DDO group showed 3,472 upregulated and 4,025 downregulated genes, whereas in the DDO group, only 145 and 72 genes were upregulated and downregulated, respectively. Conclusions Abnormal function and gene expression profiles in bladders after BOO were normalized in the BOO rats with DO after deobstruction, whereas in those without DO, abnormal function persisted and the gene expression profile became more abnormal. DO may play a protective role against the stress to the bladder induced by BOO and deobstruction as a form of adaptive neuroplasticity.

【 授权许可】

Unknown