Chronic partial urethral obstruction in female rats: description of an experimental model and initial results

Acta Cirurgica Brasileira

Silvio Tucci Jr²  Carlos Augusto Ferreira Molina¹  Marcelo Ferreira Cassini¹  Murilo Ferreira De Andrade¹  Gilson José De Lima¹  Antonio Carlos Pereira Martins¹ 

[1] ,USP FMRP Department of Surgery and Anatomy

关键词: Urethral Obstruction;    Urinary Bladder;    Rats;    Obstrução Uretral;    Bexiga Urinária;    Ratos;   

DOI  :  10.1590/S0102-86502011000800020

来源: SciELO

【 摘 要 】

PURPOSE: To develop an experimental model of infravesical urinary obstruction in female rats. METHODS: After median caudal laparotomy, the urethra of 14 female rats was delicately separated from the vagina and loosely wrapped with cellophane tape measuring 0.4 x 1.0 cm. The animals were evaluated 4 (n=7) and 8 (n=7) weeks later. Five additional control animals were only subjected to separation of the urethra and vagina and monitored for 12 weeks. RESULTS: After four weeks, three rats presented vesical dilation associated with discrete ureteral ectasis in 2 animals, with the third presenting discrete hydronephrosis in one kidney. After eight weeks, five rats (71.4%) presented vesical distension with bilateral ureterohydronephrosis. No significant changes (p>0.05) in serum urea or creatinine occurred in any group compared to preoperative values. CONCLUSION: We present here an inexpensive and simple method for the slow induction of urethral obstruction in adult female rats, with the development of progressive vesical hypertrophy and ureterohydronephrosis, which may be used as an experimental model for the study of different aspects of obstructive nephropathy.

【 授权许可】

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