Comparison of Amplification Methods to Produce Affymetrix GeneChip� Target Material

Burian D, White V, Huggins M, Kupfer D, Canfield DV, Whinnery JE

FAA Office of Aerospace Medicine - Civil Aerospace Medical Institute

关键词: Microarray Analysis;    Affymetrix GeneChip�;    RNA Amplification;    Globin-RNA Reduction;    Whole Blood;   

RP-ID  :  DOT/FAA/AM-07/9

美国|英语

来源: Federal Aviation Administration

【 摘 要 】

Whole blood from living subjects is a convenient matrix to use as a source of RNA for microarray experiments with human subjects especially when subject material is collected at a location other than the collaborating site conducting the microarray work.� Collection methods for whole blood that include stabilization of the RNA are known but suffer from issues of decreased sensitivity due to the large amount of globin RNA present from reticulocyte lysis.