Improved fermentation strategies in a bioreactor for enhancing poly(3-hydroxybutyrate) (PHB) production by wild type Cupriavidus necator from fructose

Heliyon

Oxana Yashchuk¹  Élida B. Hermida¹  Diego G. Noseda²  Beatriz Araoz³  Daiana Nygaard³ 

[1] Argentine Council of Scientific and Technical Research (CONICET), Godoy Cruz 2290, C1425FQB, CABA, Argentina;Corresponding author.;School of Science and Technology, National University of San Martín (UNSAM), Av. 25 de mayo 1147, B1650HMK, San Martín, Buenos Aires, Argentina;

关键词: Poly(3-hydroxybutyrate) (PHB);    Cupriavidus necator;    Fermentation strategies;    Optimization;    Polymer characterization;    Cytotoxicity;   

DOI  :  

来源: DOAJ

【 摘 要 】

Poly(3-hydroxybutyrate) (PHB) belongs to the family of polyhydroxyalkanoates, biopolymers used for agricultural, industrial, or even medical applications. However, scaling up the production is still an issue due to the myriad of parameters involved in the fermentation processes. The present work seeks, firstly, to scale up poly(3-hydroxybutyrate) (PHB) production by wild type C. necator ATCC 17697 from shaken flasks to a stirred-tank bioreactor with the optimized media and fructose as carbon source. The second purpose is to improve the production of PHB by applying both the batch and fed-batch fermentation strategies in comparison with previous works of wild type C. necator with fructose. Furthermore, thinking of biomedical applications, physicochemical, and cytotoxicity analyses of the produced biopolymer, are presented.Fed-batch fermentation with an exponential feeding strategy enabled us to achieve the highest values of PHB concentration and productivity, 25.7 g/l and 0.43 g/(l h), respectively. The PHB productivity was 3.3 and 7.2 times higher than the one in batch strategy and shaken flask cultures, respectively. DSC, FTIR, 1H, and 13C NMR analysis led to determine that the biopolymer produced by C. necator ATCC 17697 has a molecular structure and characteristics in agreement with the commercial PHB. Additionally, the biopolymer does not induce cytotoxic effects on the NIH/3T3 cell culture.Due to the improved fermentation strategies, PHB concentration resulted in 40 % higher of the already reported one for wild type C. necator using other fed-batch modes and fructose as a carbon source. Thus the produced PHB could be attractive for biomedical applications, which generate a rising interest in polyhydroxyalkanoates during recent years.

【 授权许可】

Unknown