Apomorphine-induced pathway perturbation in MPP+-treated SH-SY5Y cells

AIMS Molecular Science

Jin Hwan Do¹ 

[1] Department of Biomolecular and Chemical Engineering, DongYang University, Yeongju 36040, South Korea;

关键词: apomorphine;    gene expression;    structure equation model;    signaling pathway anlaysis;    SH-SY5Y cells;    Parkinson’s disease;   

DOI  :  10.3934/molsci.2017.3.271

来源: DOAJ

【 摘 要 】

Apomorphine (APOM) is a non-selective dopamine agonist for Parkinson’s disease (PD). It also offers protection against oxidative stress. Thus, it has been used for treating advanced PD patients who do not respond to levodopa or other dopamine agonists. However, side effects such as orthostatic hypotension, nausea, and fibrotic nodules at the site of APOM injection have been reported after long-term use of APOM in PD patients. To secure the use of APOM for PD treatment without side effect, it is essential to understand the molecular mechanism involved in the action of APOM in PD. In this study, gene expression profile changes by APOM in a PD cell model, i.e., MPP⁺-treated SH-SY5Y cells, were measured at six time points (0, 3, 6, 9, 12, and 24 h) after APOM treatment using a commercial whole-genome expression array. A total of 2249 genes showed significant and differential expression profile. Pathways significantly affected by APOM were estimated using signaling pathway impact analysis (SPIA). In addition, differentially regulated regions within each affected pathway were identified with covariance analysis using a structure equation model.

【 授权许可】

Unknown