Geniposide prevents rotenone-induced apoptosis in primary cultured neurons

Neural Regeneration Research

关键词: nerve regeneration;    geniposide;    rotenone;    cell apoptosis;    Alzheimer′s disease;    caspase-3;    Bcl-2;    neural regeneration;   

DOI  :  10.4103/1673-5374.167760

来源: DOAJ

【 摘 要 】

Geniposide, a monomer extracted from gardenia and widely used in Chinese medicine, is a novel agonist at the glucagon-like peptide-1 receptor. This receptor is involved in neuroprotection. In the present study, we sought to identify an anti-apoptotic mechanism for the treatment of neurodegenerative diseases. Primary cultured neurons were treated with different concentrations of rotenone for 48 hours. Morphological observation, cell counting kit-8 assay, lactate dehydrogenase detection and western blot assay demonstrated that 0.5 nM rotenone increased lactate dehydrogenase release, decreased the expression of procaspase-3 and Bcl-2, and increased cleaved caspase-3 expression in normal neurons. All these effects were prevented by geniposide. Our results indicate that geniposide diminished rotenone-induced injury in primary neurons by suppressing apoptosis. This may be one of the molecular mechanisms underlying the efficacy of geniposide in the treatment of neurodegenerative diseases.

【 授权许可】

Unknown