Long Wavelength TCF-Based Fluorescent Probe for the Detection of Alkaline Phosphatase in Live Cells

Frontiers in Chemistry

Jean-Yves Maillard¹  Gyoungmi Kim²  Juyoung Yoon²  John P. Lowe³  A. Toby A. Jenkins³  Lauren Gwynne³  George T. Williams³  Steven D. Bull³  Jordan E. Gardiner³  Tony D. James³  Adam C. Sedgwick⁴  Jonathan L. Sessler⁴ 

[1] Cardiff School of Pharmacy and Pharmaceutical Sciences, Cardiff University, Cardiff, United Kingdom;Department of Chemistry and Nano Science, Ewha Womans University, Seoul, South Korea;Department of Chemistry, University of Bath, Bath, United Kingdom;Department of Chemistry, University of Texas at Austin, Austin, TX, United States;

关键词: reaction-based fluorescent probe;    alkaline phosphatase;    cell imaging;    fluorescence;    colorimetric;   

DOI  :  10.3389/fchem.2019.00255

来源: DOAJ

【 摘 要 】

A long wavelength TCF-based fluorescent probe (TCF-ALP) was developed for the detection of alkaline phosphatase (ALP). ALP-mediated hydrolysis of the phosphate group of TCF-ALP resulted in a significant fluorescence “turn on” (58-fold), which was accompanied by a colorimetric response from yellow to purple. TCF-ALP was cell-permeable, which allowed it to be used to image ALP in HeLa cells. Upon addition of bone morphogenic protein 2, TCF-ALP proved capable of imaging endogenously stimulated ALP in myogenic murine C2C12 cells. Overall, TCF-ALP offers promise as an effective fluorescent/colorimetric probe for evaluating phosphatase activity in clinical assays or live cell systems.

【 授权许可】

Unknown