Compact, common path quantitative phase microscopic techniques for imaging cell dynamics

Pramana

R Leitgeb¹  V Trivedi²  B Javidi³  P Vora²  A Singh¹  A Anand1²  V Chhaniwal²  S Mahajan² 

[1] Center of Medical Physics and Biomedical Engineering, Medical University of Vienna, Waehringer Guertel 18-20, 4L, 1090 Vienna, Austria$$;Applied Physics Department, Faculty of Technology and Engineering, M.S. University of Baroda, Vadodara 390 001, India$$;Department of Electrical Engineering, University of Connecticut, Storrs, CT, 06269-4157, USA$$

关键词: Quantitative phase contrast imaging;    digital holography;    cell imaging;    diffraction;    three-dimensional microscopy.;   

DOI  :  

学科分类：物理（综合）

来源: Indian Academy of Sciences

【 摘 要 】

Microscopy using visible electromagnetic radiation can be used to investigate living cells in various environments. But bright field microscopy only provides two-dimensional (2D) intensity distribution at a single object plane. One of the ways to retrieve object height/thickness information is to employ quantitative phase microscopic (QPM) techniques. Interferometric QPM techniques are widely used for this. Digital holographic microscopy (DHM) is one of the stateof-the-art methods for quantitative three-dimensional (3D) imaging. Usually it is implemented in two-beam geometry, which is prone to mechanical vibrations. But to study dynamics of objects like red blood cells, one needs temporal stability much better than the fluctuations of the object, which the two-beam geometry fails to deliver. One way to overcome this hurdle is to use selfreferencing techniques, in which a portion of the object beam will act as the reference beam. Here the development of self-referencing QPM techniques is described along with the results.

【 授权许可】

Unknown