A Novel Dental Sealant Containing Dimethylaminohexadecyl Methacrylate Suppresses the Cariogenic Pathogenicity of Streptococcus mutans Biofilms

International Journal of Molecular Sciences

NancyJ. Lin¹  ThomasW. Oates²  FranklinR. Tay³  AhmedS. Ibrahim⁴  AbdulrahmanA. Balhaddad⁵  HockinH. K. Xu⁵  MariaSalem Ibrahim⁵  MichaelD. Weir⁵  MaryAnne S. Melo⁵ 

[1] Biosystems and Biomaterials Division, National Institute of Standards and Technology, Gaithersburg, MD 20899, USA;Department of Advanced Oral Sciences and Therapeutics, Division of Biomaterials and Tissue Engineering, University of Maryland School of Dentistry, Baltimore, MD 21201, USA;Department of Endodontics, The Dental College of Georgia, Augusta University, Augusta, GA 30912, USA;Medical Microbiology Department, Health Monitoring Centers, Ministry of Health, Jeddah 21176, Saudi Arabia;Ph.D Program in Dental Biomedical Sciences, University of Maryland School of Dentistry, Baltimore, MD 21201, USA;

关键词: antibacterial agents;    biofilm;    dental caries;    sealant;    quaternary ammonium compounds;    Streptococcus mutans;   

DOI  :  10.3390/ijms20143491

来源: DOAJ

【 摘 要 】

Cariogenic oral biofilms are strongly linked to dental caries around dental sealants. Quaternary ammonium monomers copolymerized with dental resin systems have been increasingly explored for modulation of biofilm growth. Here, we investigated the effect of dimethylaminohexadecyl methacrylate (DMAHDM) on the cariogenic pathogenicity of Streptococcus mutans (S. mutans) biofilms. DMAHDM at 5 mass% was incorporated into a parental formulation containing 20 mass% nanoparticles of amorphous calcium phosphate (NACP). S. mutans biofilms were grown on the formulations, and biofilm inhibition and virulence properties were assessed. The tolerances to acid stress and hydrogen peroxide stress were also evaluated. Our findings suggest that incorporating 5% DMAHDM into 20% NACP-containing sealants (1) imparts a detrimental biological effect on S. mutans by reducing colony-forming unit counts, metabolic activity and exopolysaccharide synthesis; and (2) reduces overall acid production and tolerance to oxygen stress, two major virulence factors of this microorganism. These results provide a perspective on the value of integrating bioactive restorative materials with traditional caries management approaches in clinical practice. Contact-killing strategies via dental materials aiming to prevent or at least reduce high numbers of cariogenic bacteria may be a promising approach to decrease caries in patients at high risk.

【 授权许可】

Unknown