Anti-Biofilm Activity of Cannabigerol against Streptococcus mutans

Microorganisms

Muna Aqawi¹  Ronit Vogt Sionov¹  Doron Steinberg¹  Michael Friedman²  Ruth Gallily³ 

[1] The Biofilm Research Laboratory, The Faculty of Dental Medicine, The Institute of Dental Sciences, The Hebrew University of Jerusalem, Jerusalem 9112102, Israel;The Institute of Drug Research, School of Pharmacy, Faculty of Medicine, The Hebrew University of Jerusalem, Jerusalem 9112102, Israel;The Lautenberg Center for General and Tumor Immunology, The Hadassah Medical School, The Hebrew University of Jerusalem, Jerusalem 9112102, Israel;

关键词: biofilm;    Cannabigerol;    dental caries;    Streptococcus mutans;   

DOI  :  10.3390/microorganisms9102031

来源: DOAJ

【 摘 要 】

Streptococcus mutans is a common cariogenic bacterium in the oral cavity involved in plaque formation. Previous studies showed that Cannabigerol (CBG) has bacteriostatic and bacteriocidic activity against S. mutans. The aim of the present study was to study its effect on S. mutans biofilm formation and dispersion. S. mutans was cultivated in the presence of CBG, and the resulting biofilms were examined by CV staining, MTT assay, qPCR, biofilm tracer, optical profilometry, and SEM. Gene expression was determined by real-time qPCR, extracellular polysaccharide (EPS) production was determined by Congo Red, and reactive oxygen species (ROS) were determined using DCFH-DA. CBG prevented the biofilm formation of S. mutans shown by reduced biofilm biomass, decreased biofilm thickness, less EPS production, reduced DNA content, diminished metabolic activity, and increased ROS levels. CBG altered the biofilm roughness profile, resulting in a smoother biofilm surface. When treating preformed biofilms, CBG reduced the metabolic activity of S. mutans with a transient effect on the biomass. CBG reduced the expression of various genes involved in essential metabolic pathways related to the cariogenic properties of S. mutans biofilms. Our data show that CBG has anti-biofilm activities against S. mutans and might be a potential drug for preventive treatment of dental caries.

【 授权许可】

Unknown