Isolation and Characterization of Streptococcus mutans Phage as a Possible Treatment Agent for Caries

Viruses

Gilad Ben-Gal¹  Reut Kraitman¹  Nurit Beyth¹  Hadar Ben-Zaken¹  Shunit Coppenhagen-Glazer²  Ronen Hazan²  Sivan Alkalay-Oren²  Leron Khalifa²  Daniel Gelman² 

[1] Department of Prosthodontics, Hadassah School of Dental Medicine, Hebrew University, Jerusalem 91120, Israel;Institute of Biomedical and Oral Research (IBOR), Faculty of Dental Sciences, School of Dental Medicine, The Hebrew University, Jerusalem 91120, Israel;

关键词: S. mutans;    phage therapy;    dental caries;    S. mutans phage;    bacteriophage;    biofilm;   

DOI  :  10.3390/v13050825

来源: DOAJ

【 摘 要 】

Streptococcus mutans is a key bacterium in dental caries, one of the most prevalent chronic infectious diseases. Conventional treatment fails to specifically target the pathogenic bacteria, while tending to eradicate commensal bacteria. Thus, caries remains one of the most common and challenging diseases. Phage therapy, which involves the use of bacterial viruses as anti-bacterial agents, has been gaining interest worldwide. Nevertheless, to date, only a few phages have been isolated against S. mutans. In this study, we describe the isolation and characterization of a new S. mutans phage, termed SMHBZ8, from hundreds of human saliva samples that were collected, filtered, and screened. The SMHBZ8 genome was sequenced and analyzed, visualized by TEM, and its antibacterial properties were evaluated in various states. In addition, we tested the lytic efficacy of SMHBZ8 against S. mutans in a human cariogenic dentin model. The isolation and characterization of SMHBZ8 may be the first step towards developing a potential phage therapy for dental caries.

【 授权许可】

Unknown