A Comparative Study of the Effects of Anticoagulants on Pure Platelet-Rich Plasma Quality and Potency

Biomedicines

Koh Nakata¹  Tomoyuki Kawase²  Masayuki Nakamura³  Hideo Kawabata³  Kazushige Isobe³  Taisuke Watanabe³  Hachidai Aizawa³  Hideo Masuki³  Tetsuhiro Tsujino³  Atsushi Sato³ 

[1] Bioscience Medical Research Center, Niigata University Medical and Dental Hospital, Niigata 951-8520, Japan;Division of Oral Bioengineering, Institute of Medicine and Dentistry, Niigata University, Niigata 951-8514, Japan;Tokyo Plastic Dental Society, Kita-ku, Tokyo 114-0002, Japan;

关键词: anticoagulants;    edta;    acd-a;    heparin;    platelets;    platelet-rich plasma;    pdgf;   

DOI  :  10.3390/biomedicines8030042

来源: DOAJ

【 摘 要 】

It is generally accepted that citrate or the A-form of acid-citrate-dextrose (ACD-A) are suitable for preparing platelet-rich plasma (PRP) for regenerative therapy. However, this is based on evidence from blood transfusions and not from regenerative medicine. Thus, we examined the effects of anticoagulants, such as ACD-A, ethylenediaminetetraacetic acid (EDTA), and heparin, on the regenerative quality of PRP to address this gap. The blood samples were collected in the presence of anticoagulants and were processed to prepare pure-PRP. Platelet size, activation status, and intra-platelet free Ca²⁺ concentration were determined while using a hematology analyzer and flow cytometer. Platelet-derived growth factor-BB (PDGF-BB) was quantified while using an ELISA. In pure-PRP samples, EDTA caused platelet swelling and activation, but yielded the highest number of platelets. Heparin aggregated platelets and disturbed the overall counting of blood cells. However, no significant differences in PDGF-BB levels were observed among the anticoagulants tested. Moreover, when considering the easy preparation of platelet suspensions, without the need for high-level pipetting skills, these findings suggest the comparable potency of EDTA-derived pure-PRP in tissue regeneration and support the use of EDTA in the preparation of pure-PRP. Further in vivo studies are required in animal models to exclude the possible negative effects of including EDTA in pure-PRP preparations.

【 授权许可】

Unknown