Bone Marrow-Derived Stem Cell Populations Are Differentially Regulated by Thyroid or/and Ovarian Hormone Loss

International Journal of Molecular Sciences

Eltyeb Abdelwahid¹  Carolina M. C. O. de Souza¹  Rossana B. Simeoni¹  Luiz C. Guarita-Souza¹  Bassam F. Mogharbel²  Daiany de Souza²  Katherine A. T. de Carvalho²  Julio C. Francisco²  Reginaldo J. Ferreira²  Míriam P. Beltrame³  Ana C. Irioda⁴ 

[1] Pequeno Príncipe Faculty, Ave. Silva Jardim 1632, Box 80.250-200 Curitiba, Paraná, Brazil;;Cell Therapy and Biotechnology in Regenerative Medicine Department, The Pelé Pequeno Príncipe Institute, Child and Adolescent Health Research &Experimental Laboratory of Institute of Biological and Health Sciences of Pontifical Catholic University of Paraná (PUCPR), Rua Imaculada Conceição, 1155, Box 80.215-901 Curitiba, Paraná, Brazil;Feinberg School of Medicine, Feinberg Cardiovascular Research Institute, Northwestern University, 303 E. Chicago Ave., Tarry 14-725, Chicago, IL 60611, USA;

关键词: hypothyroidism;    estrogen;    precursor B cells/Pro-B cell;    mesenchymal stem cell;    endothelial progenitor cell;    bone marrow;    ovariectomy;    thyroidectomy;   

DOI  :  10.3390/ijms18102139

来源: DOAJ

【 摘 要 】

Bone marrow-derived stem cells (BMDSCs) play an essential role in organ repair and regeneration. The molecular mechanisms by which hormones control BMDSCs proliferation and differentiation are unclear. Our aim in this study was to investigate how a lack of ovarian or/and thyroid hormones affects stem cell number in bone marrow lineage. To examine the effect of thyroid or/and ovarian hormones on the proliferative activity of BMDSCs, we removed the thyroid or/and the ovaries of adult female rats. An absence of ovarian and thyroid hormones was confirmed by Pap staining and Thyroid Stimulating Hormone (TSH) measurement, respectively. To obtain the stem cells from the bone marrow, we punctured the iliac crest, and aspirated and isolated cells by using a density gradient. Specific markers were used by cytometry to identify the different BMDSCs types: endothelial progenitor cells (EPCs), precursor B cells/pro-B cells, and mesenchymal stem cells (MSCs). Interestingly, our results showed that hypothyroidism caused a significant increase in the percentage of EPCs, whereas a lack of ovarian hormones significantly increased the precursor B cells/pro-B cells. Moreover, the removal of both glands led to increased MSCs. In conclusion, both ovarian and thyroid hormones appear to have key and diverse roles in regulating the proliferation of cells populations of the bone marrow.

【 授权许可】

Unknown