CD301b+ macrophages mediate angiogenesis of calcium phosphate bioceramics by CaN/NFATc1/VEGF axis

Bioactive Materials

Yufeng Zhang¹  Qin Zhao¹  Litian Han¹  Shihang Zheng¹  Ziming Wang¹  Jiaolong Wang²  Zijian Gong²  Liangliang Fu²  Can Wang²  Hua Tang² 

[1] Hospital of Stomatology, Wuhan University, Wuhan, 430079, Hubei, PR China;;The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) and Key Laboratory of Oral Biomedicine, Ministry of Education, School &

关键词: Calcium phosphate ceramics;    Macrophage;    Angiogenesis;    CD301b;    Calcineurin;   

DOI  :  

来源: DOAJ

【 摘 要 】

Calcium phosphate (CaP) bioceramics are important for tissue regeneration and immune response, yet how CaP bioceramics influence these biological processes remains unclear. Recently, the role of immune cells in biomaterial-mediated regeneration, especially macrophages, has been well concerned. CD301b+ macrophages were a new subset of macrophages we have discovered, which were required for bioceramics-mediated bone regeneration. Nevertheless, the impact of CD301b+ macrophages on angiogenesis, which is a vital prerequisite to bone formation is yet indistinct. Herein, we found that CD301b+ macrophages were closely correlated to angiogenesis of CaP bioceramics. Additionally, depletion of CD301b+ macrophages led to the failure of angiogenesis. We showed that store-operated Ca2+ entry and calcineurin signals regulated the VEGF expression of CD301b+ macrophages via the NFATc1/VEGF axis. Inhibition of calcineurin effectively impaired angiogenesis via decreasing the infiltration of CD301b+ macrophages. These findings provided a potential immunomodulatory strategy to optimize the integration of angiogenesis and bone tissue engineering scaffold materials.

【 授权许可】

Unknown