In vitro biological activities of silver nanoparticles synthesized from Scedosporium sp. isolated from soil

Brazilian Journal of Pharmaceutical Sciences

关键词: Atomic force microscopy;    In vitro cytotoxicity;    Scedosporium sp;    Silver nanoparticles;    UV-visible spectroscopy;   

DOI  :  10.1590/s2175-97902019000200254

来源: DOAJ

【 摘 要 】

One of the important fields in nanotechnology is the development of an environment friendly method for the synthesis of nanoparticles. Many approaches show that microorganisms are the most reliable tools for biosynthesis of nanoparticles compared to physical and chemical methods. In our study, fungi have been exploited for extracellular production of metal nanoparticles. It was observed that in Scedosporium, silver ions are reduced to silver nanoparticles, which was confirmed by UV-visible spectrophotometry and AFM. Optimization studies showed that as the concentration of AgNO3 used for synthesis increased, particles’ size also increased. Size of the particles at different concentrations of AgNO3 was observed to be 79-107 nm with particles being ellipsoidal to spherical in shape. Silver nanoparticles synthesized from 2.0 mM silver nitrate, showed maximum antimicrobial activity compared to all antibiotics tested including synergistic effects. In vitro cytotoxicity of silver nanoparticles against MCF 7 and PC 3 showed that as the concentration of silver nanoparticles increased, a decrease in the percentage cell viability was observed with IC50 values being 60.09 and 57.43 µg/ml respectively. Therefore, through this study, it could be said that extracellular synthesis of silver nanoparticles from Scedosporium was simple, ecofriendly, proving excellent antimicrobial and anticancer agents.

【 授权许可】

Unknown