Tracking neuronal motility in live murine retinal explants

STAR Protocols

Katherine M. Warre-Cornish¹  Robin R. Ali²  Rachael A. Pearson²  Martha R. Robinson²  Nozie D. Aghaizu² 

[1] Corresponding author;University College London, Institute of Ophthalmology, London EC1V 9EL, UK;

关键词: Cell Biology;    Developmental biology;    Microscopy;    Model Organisms;    Neuroscience;   

DOI  :  

来源: DOAJ

【 摘 要 】

Summary: The developing retina undergoes dynamic organizational changes involving significant intra-retinal motility of the encompassing cells. Here, we present a protocol for tracking retinal cell motility in live explanted mouse retinae. Although originally applied to rod and cone photoreceptors, this strategy is applicable to any fluorescently labeled cell in mouse retinae and other similar experimental retinal models. Careful tissue handling is critical for the successful acquisition of high-quality live imaging data. Further instructions for semi-automated in silico data handling are provided.For complete details on the use and execution of this protocol, please refer to Aghaizu et al. (2021).

【 授权许可】

Unknown