Protocol for ex vivo time lapse imaging of Drosophila melanogaster cytonemes

STAR Protocols

Thomas B. Kornberg¹  Guilherme O. Barbosa² 

[1] Corresponding author;Cardiovascular Research Institute, University of California, San Francisco, San Francisco, CA, USA;

关键词: Cell Biology;    Developmental biology;    Microscopy;    Model Organisms;   

DOI  :  

来源: DOAJ

【 摘 要 】

Summary: This protocol describes how to image time and spatially resolved time lapses of Drosophila air sac primordium (ASP) cytonemes in ex vivo cultures of wing imaginal discs. It describes how to manually measure the length of cytonemes using custom-made FIJI/ImageJ tools, and to analyze data using R/R-Studios pipeline. It can also be used for studies of cell division, organelle localization, and protein trafficking as well as other cellular materials that can be fluorescently tagged and imaged with minimal phototoxicity.

【 授权许可】

Unknown