Analysis of proteostasis during aging with western blot of detergent-soluble and insoluble protein fractions

STAR Protocols

Fabio Demontis¹  Jianqin Jiao¹  Michelle Curley¹  Flavia A. Graca¹  Mamta Rai¹  Liam C. Hunt¹  Zane Coleman¹  Anjana Nityanandam² 

[1] Department of Developmental Neurobiology, St. Jude Children’s Research Hospital, Memphis, TN 38105, USA;Stem Cell Core, Department of Developmental Neurobiology, St. Jude Children’s Research Hospital, Memphis, TN 38105, USA;

关键词: Cell Biology;    Developmental biology;    Model Organisms;    Molecular Biology;    Protein Biochemistry;    Organoids;   

DOI  :  

来源: DOAJ

【 摘 要 】

Summary: Defects in protein quality control are the underlying cause of age-related diseases. The western blot analysis of detergent-soluble and insoluble protein fractions has proven useful in identifying interventions that regulate proteostasis. Here, we describe the protocol for such analyses in Drosophila tissues, mouse skeletal muscle, human organoids, and HEK293 cells. We describe key adaptations of this protocol and provide key information that will help modify this protocol for future studies in other tissues and disease models.For complete details on the use and execution of this protocol, please refer to Rai et al. (2021) and Hunt el al. (2021).

【 授权许可】

Unknown