Genetic mosaic dissection of candidate genes in mice using mosaic analysis with double markers

STAR Protocols

Simon Hippenmeyer¹  Nicole Amberg¹ 

[1] Institute of Science and Technology Austria, Am Campus 1, 3400 Klosterneuburg, Austria;

关键词: Single Cell;    Developmental biology;    Genetics;    Microscopy;    Model Organisms;    Molecular Biology;   

DOI  :  

来源: DOAJ

【 摘 要 】

Summary: Mosaic analysis with double markers (MADM) technology enables the generation of genetic mosaic tissue in mice. MADM enables concomitant fluorescent cell labeling and introduction of a mutation of a gene of interest with single-cell resolution. This protocol highlights major steps for the generation of genetic mosaic tissue and the isolation and processing of respective tissues for downstream histological analysis.For complete details on the use and execution of this protocol, please refer to Contreras et al. (2021).

【 授权许可】

Unknown