Green synthesis of gold nanoparticles using Jatropha integerrima Jacq. flower extract and their antibacterial activity

Journal of King Saud University: Science

Khaloud Mohammed Alarjani¹  Dina S. Hussein²  Rabab Ahmed Rasheed³  K. Kanimozhi⁴  Sivaji Sathiyaraj⁵  Gunasekaran Suriyakala⁵  Ranganathan Babujanarthanam⁵ 

[1] Corresponding author.;Department of Botany and Microbiology, College of Science, King Saud University, Riyadh 11451, Saudi Arabia;Department of Chemistry, College of Sciences and Health, Cleveland State University, Cleveland 44115, United States;;Histology &Nano and Energy Bioscience Laboratory, Department of Biotechnology, Thiruvalluvar University, Serkkadu, Vellore 632115, Tamil Nadu, India;

关键词: Gold nanoparticles;    Jatropha integerrima Jacq.;    TEM;    Antibacterial activity;    Escherichia coli;   

DOI  :  

来源: DOAJ

【 摘 要 】

Background: With the rise of antibiotic resistance, there is an increasing interest in discovering novel antimicrobial agents. Antibiotics could be replaced with metal-based nanoparticles that have long-lasting antibacterial properties. This study aimed to synthesize gold nanoparticles (AuNPs) and test their antibacterial effectiveness against a variety of human pathogens. Methods: The AuNPs were characterized using UV, FT-IR, XRD, and TEM with EDX. By using the well diffusion and microdilution techniques, the impact of synthesized AuNPs was tested against B. subtilis, S. aureus, E. coli, and K. pneumoniae. Results: The AuNPs were synthesized from Jatropha integerrima Jacq. flower extract. UV–vis spectrum showed a high peak at 547 nm; FT-IR revealed phenolic compounds in the plant extract were responsible for AuNP formation. XRD and SAED confirmed the crystalline nature while TEM revealed the shape to be spherical and DLS revealed the size to be 38.8 nm with the stability of −0.3 mV. The AuNPs exhibit maximal and minimal antibacterial activity towards E. coli and B. subtilis. The MIC of AuNPs against B. subtilis, S. aureus, E. coli, and K. pneumoniae were found to be 5.0, 10, 2.5, and 2.5 μg/mL, respectively. Conclusion: Thus, synthesized nanoparticles might be a good alternative to develop an antibacterial agent against the selected human pathogens.

【 授权许可】

Unknown