Efficient strategy to isolate exosomes using anti-CD63 antibodies conjugated to gold nanoparticles

AMB Express

Original Article

Lavleen Kumar Gupta¹  Arvind Kumar¹  N. S. Sampath Kumar²  Anjani Devi Chintagunta²  Deepali Shrivastava²  Dikshita Panwar² 

[1] IgY Immunologix India Private Limited, Narsingi, Rangareddy, 500089, Hyderabad, Telangana, India;Vignan’s Foundation for Science, Technology and Research, Guntur -Tenali Rd, 522213, Vadlamudi, Andhra Pradesh, India;

关键词: IgY@AuNPs;    Extracellular vesicles;    Exosomes;    ELISA;    TEM;    Gold nanoparticles;    Tetraspanins;   

DOI  :  10.1186/s13568-023-01592-1

 received in 2023-05-10, accepted in 2023-08-03,  发布年份 2023

来源: Springer

【 摘 要 】

Exosomes, a subpopulation of Extracellular vesicles (EVs), are cell-secreted vesicles found in the majority of biological fluids, including breast milk, tears, sweat, blood and, urine. The density and size of these vesicles depend on a variety of factors, including age, gender and the biological condition of the individual. Researchers are now focusing on the selective extraction of exosomes from bodily fluids due to the unique biomolecule composition of exosomes, which is critical for diagnosis, disease, and regeneration. Furthermore, current approaches for exosome isolation have limitations, necessitating the development of a simpler and more effective technique to achieve this goal. In this study, we investigated a quick and effective strategy for isolating exosomes from serum using a bench-top centrifuge. This was accomplished by raising antibodies against exosome surface tetraspanins (CD9, CD63 & CD81) in Leghorn chickens due to their phylogenetic distance from humans and cost-effectiveness for commercial use. In order to separate exosomes from a complex biological fluid, the antibodies were further coupled with gold nanoparticles (AuNPs). The findings were validated using ELISA, spectrophotometry, and transmission electron microscopy (TEM). Using this technique, exosome isolation from serum was achieved rapidly and these were captured by using anti CD63 antibodies bound to AuNPs. To summarize, exosomes were purified from serum using anti-CD63 antibodies conjugated to gold nanoparticles (IgY@AuNPs). Consequently, the approach for exosome isolation from biological fluid could be useful for clinically monitoring the biological state of the patients.

【 授权许可】

CC BY   
© Springer-Verlag GmbH Germany, part of Springer Nature 2023

【 图 表 】

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