Optimization of the process for purifying icariin from Herba Epimedii by macroporous resin and the regulatory role of icariin in the tumor immune microenvironment

Biomedicine & Pharmacotherapy

Dihua Li¹  Lanqiu Zhang²  Caixia Li²  Ximo Wang³  Xin Zheng³  Botao Wang³  Lei Yang³  Xiangfei Yuan³  Lihua Cui³  Jiaxin Li³  Qi Zhang³ 

[1] Department of General Surgery, The 2nd Affiliated Hospital of Chengdu Medical College, Nuclear Industry 416 Hospital, Chengdu, 610051, China;Graduate School, Tianjin Medical University, Tianjin, 300070, China;Tianjin Key Laboratory of Acute Abdominal Disease-Associated Organ Injury and ITCWM Repair, Institute ofAcute Abdominal Diseases, Tianjin Nankai Hospital, Tianjin, 300100, China;

关键词: Icariin;    Macroporous resin;    Herba Epimedii;    Pancreatic cancer;    Immune microenvironment;   

DOI  :  

来源: DOAJ

【 摘 要 】

Pancreatic cancer is a digestive tract malignancy that poses a serious threat to human health. Compounds derived from traditional Chinese medicines have been an important source of anticancer drugs and adjuvant agents to regulate the tumor immune microenvironment in patients with pancreatic cancer. In this study, icariin was purified from Herba Epimedii using macropores, and its bioactivity against pancreatic cancer was also investigated. We found that icariin has direct inhibitory and immunomodulatory effects on tumor cells. In vitro experiments showed that icariin can inhibit the migration and proliferation of Panc02 pancreatic cancer cells and induce apoptosis. Our in vivo experiments show that icariin inhibits the development of mouse pancreatic cancer by inhibiting tumor-infiltrating M2 macrophages and polymorphonuclear myeloid-derived suppressor cells (MDSCs) (PMN-MDSCs). In addition, icariin inhibits the polarization of RAW 264.7 cells into M2 macrophages by inhibiting the expression of ARG1 and MRC1 and downregulating the IL4-STAT6 signaling pathway. In conclusion, the inhibitory effect of icariin on pancreatic cancer can not only directly affect tumor cells but also inhibit tumor development by regulating the tumor immune microenvironment.

【 授权许可】

Unknown