Study on Crude Flavonoids Extraction from Longan Leaves and its anti Human Lung Adenocareinoma Cell Line A549

2nd International Symposium on Resource Exploration and Environmental Science

生态环境科学

Zheng, Wei^1 ; Dong, Xueming^1 ; Zhang, Qiuying^1 ; Yu, Xuefei^2

Research Center on Life Sciences and Environmental Sciences, Harbin University of Commerce, Harbin

150076, China^1

Library, Harbin University of Commerce, Harbin

150028, China^2

关键词: Cell-shrinkage;    Crude extract;    Dry weight;    Extraction and purifications;    Macroporous resin;    Morphological changes;    Nuclear fragmentation;    Total flavonoids;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/170/2/022147/pdf
DOI  :  10.1088/1755-1315/170/2/022147

学科分类：环境科学（综合）

来源: IOP

【 摘 要 】

The crude extracts of flavonoids were obtained from longan leaves via ultrasonic and purified with macroporous resin of AB-8. Taking rutin as the standard, the amount of flavonoids in crude extracts was measured by ultraviolet spectrophotometer. The concentration of flavonoids after extraction and purification was 0.775 μg/mL. Total flavonoids in the extractum accounted for 77.5%. Total flavonoids accounted for 77.49% of the dry weight of the longan leaves. MTT assay was applied to test the A549 cell activities treated by a series of concentrations of flavonoids (50, 100, 200, 400 and 800 μg/mL) for 24h, 48h and 72h, respectively. The morphological changes of A549 cells treated by 100, 200 and 400μg/mL flavonoids for 48h were observed by inverted microscope and fluorescence microscope, respectively. The results showed that the total flavonoids of longan leaves obviously inhibit the proliferation of A549 cells, with cell shrinkage, nuclear fragmentation and apoptosis occurred.