Antibacterial activities of the methanol extract, fractions and compounds from Elaeophorbia drupifera (Thonn.) Stapf. (Euphorbiaceae)

BMC Complementary and Alternative Medicine

Research Article

Igor K. Voukeng¹  Victor Kuete¹  Veronique P. Beng²  Ilhami Celik³  Pierre Tane⁴  Blaise K. Nganou⁴  Louis P. Sandjo⁵ 

[1] Department of Biochemistry, Faculty of Science, University of Dschang, P.O. Box 67, Dschang, Cameroon;Department of Biochemistry, Faculty of Science, University of Yaounde I, Yaoundé, Cameroon;Department of Chemistry, Faculty of Science, Anadolu University, Eskişehir, Turkey;Department of Chemistry, Faculty of Science, University of Dschang, Dschang, Cameroon;Department of Pharmaceutical Sciences, CCS, Universidade Federal de Santa Catarina, 88040-900, Florianópolis, Santa Catarina, Brazil;

关键词: Antibacterial;    Crude extract;    Compounds;    Elaeophorbia drupifera;    Fractions;    Multi-drug resistance;   

DOI  :  10.1186/s12906-016-1509-y

 received in 2016-08-26, accepted in 2016-12-02,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundElaeophorbia drupifera (Thonn.) Stapf. (Euphorbiaceae) is used in Cameroonian folk medicine to treat several ailments including bacterial-related diseases such as skin infections. In this study, the methanol extract from the leaves (EDL), fractions (EDLa-d), sub-fractions EDLc1-7 and EDLc31-35 as well as isolated compounds were tested for their antimicrobial activities against a panel of Gram-negative and Gram-positive bacteria including multidrug resistant (MDR) phenotypes.MethodsThe broth microdilution method was used to determine the minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) of the above samples; column chromatography was used for the fractionation and purification of the leaves extract whilst the chemical structures of compounds were determined using spectroscopic techniques.ResultsPhytochemical investigation lead to the isolation of a mixture (1:3) of stigmasterol and β-sitosterol (1 + 2), euphol (3), sitosterol-O-β-D-xylopyranoside (4), 3,3′,4′-tri-O-methylellagic acid (5), a mixture (1:1) of afzelin and quercetin-3-O-β-D-xylopyranoside (6 + 7), 3,3′,4′-tri-O-methylellagic acid 4-O-β-D-glucopyranoside (8), ellagic acid-4-O-β-xylopyranoside-3,3′,4′-trimethyl ether (9) from EDLc. Crude extract and fractions displayed selective activities with MIC values ranged from 32 to 1024 μg/mL for EDL against 84.9% of the 33 tested bacteria, 93.9% for EDLc, 69.7% for EDLb, 33.4% for EDLa and 0.03% for EDLd. MIC values ranged from 16 to 1024 μg/mL were obtained with EDLc3 and EDLc4 on all tested bacteria meanwhile other sub-fractions displayed selective activities. MIC value of 32 μg/mL was obtained with fractions EDLa against Escherichia coli AG100, EDLc against Enterobacer aerogenes ATCC13048 and EA298. For sub-fractions obtained from EDLc, the lowest MIC value of 16 μg/mL was recorded with EDLc3 against Staphylococcus aureus MRSA11. A corresponding value of 8 μg/mL against Providencia stuartii NAE16 was recorded with EDLc33 obtained from further fractionation of EDLc3. EDLc3 had MIC values below 100 μg/mL against all tested bacteria. Compound 5 as well as the mixture (1:1) of 6 and 7 inhibited the growth of all the tested bacteria with MICs ranged from 64 to 256 μg/mL.ConclusionElaeophorbia drupifera is a potential source of phytomedicine to tackle MDR bacteria. Sub-fraction EDLc3 was more active than all isolated compounds and deserves further investigations to develop natural drug to combat Gram-negative, Gram-positive bacteria and otherwise MDR phenotypes.

【 授权许可】

CC BY   
© The Author(s). 2017