High Sensitivity Detection of CdSe/ZnS Quantum Dot-Labeled DNA Based on N-type Porous Silicon Microcavities

Sensors

Zhenhong Jia¹  Jie Lv²  Yanyu Li³  Hongyan Zhang³  Changwu Lv³ 

[1] College of Information Science and Engineering, Xinjiang University, Urumqi 830046, China;College of Resource and Environment sciences, Xinjiang University, Urumqi 830046, China;School of Physical Science and Technology, Xinjiang University, Urumqi 830046, China;

关键词: N-type porous silicon;    quantum dot labeling;    QD-DNA;    reflectance spectrum;   

DOI  :  10.3390/s17010080

来源: DOAJ

【 摘 要 】

N-type macroporous silicon microcavity structures were prepared using electrochemical etching in an HF solution in the absence of light and oxidants. The CdSe/ZnS water-soluble quantum dot-labeled DNA target molecules were detected by monitoring the microcavity reflectance spectrum, which was characterized by the reflectance spectrum defect state position shift resulting from changes to the structures’ refractive index. Quantum dots with a high refractive index and DNA coupling can improve the detection sensitivity by amplifying the optical response signals of the target DNA. The experimental results show that DNA combined with a quantum dot can improve the sensitivity of DNA detection by more than five times.

【 授权许可】

Unknown