Beads-Based Electrochemical Assay for the Detection of Influenza Hemagglutinin Labeled with CdTe Quantum Dots

Molecules

Ludmila Krejcova¹  Lukas Nejdl¹  David Hynek¹  Sona Krizkova¹  Pavel Kopel¹  Vojtech Adam¹ 

[1] Department of Chemistry and Biochemistry, Faculty of Agronomy, Mendel University in Brno, Zemedelska 1, Brno CZ-613 00, Czech Republic

关键词: influenza hemagglutinin;    electrochemical detection;    paramagnetic particles;    glycan;    quantum dot labeling;   

DOI  :  10.3390/molecules181215573

来源: mdpi

【 摘 要 】

In this study we describe a beads-based assay for rapid, sensitive and specific isolation and detection of influenza vaccine hemagglutinin (HA). Amplification of the hemagglutinin signal resulted from binding of an electrochemical label as quantum dots (QDs). For detection of the metal and protein part of the resulting HA-CdTe complex, two differential pulse voltammetric methods were used. The procedure includes automated robotic isolation and electrochemical analysis of the isolated product. The isolation procedure was based on the binding of paramagnetic particles (MPs) with glycan (Gly), where glycan was used as the specific receptor for linkage of the QD-labeled hemagglutinin.

【 授权许可】

CC BY   
© 2013 by the authors; licensee MDPI, Basel, Switzerland.