A Recombinant HAV Expressing a Neutralization Epitope of HEV Induces Immune Response against HAV and HEV in Mice

Viruses

Guan Ying¹  Yi Shan²  Sun Maosheng²  Li Hongjun²  Zhou Yan²  Wu Jinyuan²  Yin Na²  Kui Xiang²  Wang Yan³  Yuri Kusov⁴ 

[1] China Tobacco Yunnan Industrial Co., Ltd., 41 Keyi Road, Kunming 650106, China;Department of Molecular Biology, Institute of Medical Biology, Chinese Academy of Medical Sciences, 935 Jiaoling Road, Kunming 650118, China;Department of Pathology, The Second Affiliated Hospital of Kunming Medical University, 374 Dianmian Road, Kunming 650101, China;Institute of Biochemistry, Center for Structural and Cell Biology in Medicine, University of Lübeck, Ratzeburger Allee 160, 23562 Lübeck, Germany;

关键词: combined vaccine;    hepatitis A;    hepatitis E;    virus;    vector;    neutralization epitope;   

DOI  :  10.3390/v9090260

来源: DOAJ

【 摘 要 】

Hepatitis A virus (HAV) and hepatitis E virus (HEV) are causative agents of acute viral hepatitis transmitted via the fecal–oral route. Both viruses place a heavy burden on the public health and economy of developing countries. To test the possibility that HAV could be used as an expression vector for the development of a combination vaccine against hepatitis A and E infections, recombinant HAV-HEp148 was created as a vector to express an HEV neutralization epitope (HEp148) located at aa 459–606 of the HEV capsid protein. The recombinant virus expressed the HEp148 protein in a partially dimerized state in HAV-susceptible cells. Immunization with the HAV-HEp148 virus induced a strong HAV- and HEV-specific immune response in mice. Thus, the present study demonstrates a novel approach to the development of a combined hepatitis A and E vaccine.

【 授权许可】

Unknown