Viruses | |
A Recombinant HAV Expressing a Neutralization Epitope of HEV Induces Immune Response against HAV and HEV in Mice | |
Guan Ying1  Yi Shan2  Sun Maosheng2  Li Hongjun2  Zhou Yan2  Wu Jinyuan2  Yin Na2  Kui Xiang2  Wang Yan3  Yuri Kusov4  | |
[1] China Tobacco Yunnan Industrial Co., Ltd., 41 Keyi Road, Kunming 650106, China;Department of Molecular Biology, Institute of Medical Biology, Chinese Academy of Medical Sciences, 935 Jiaoling Road, Kunming 650118, China;Department of Pathology, The Second Affiliated Hospital of Kunming Medical University, 374 Dianmian Road, Kunming 650101, China;Institute of Biochemistry, Center for Structural and Cell Biology in Medicine, University of Lübeck, Ratzeburger Allee 160, 23562 Lübeck, Germany; | |
关键词: combined vaccine; hepatitis A; hepatitis E; virus; vector; neutralization epitope; | |
DOI : 10.3390/v9090260 | |
来源: DOAJ |
【 摘 要 】
Hepatitis A virus (HAV) and hepatitis E virus (HEV) are causative agents of acute viral hepatitis transmitted via the fecal–oral route. Both viruses place a heavy burden on the public health and economy of developing countries. To test the possibility that HAV could be used as an expression vector for the development of a combination vaccine against hepatitis A and E infections, recombinant HAV-HEp148 was created as a vector to express an HEV neutralization epitope (HEp148) located at aa 459–606 of the HEV capsid protein. The recombinant virus expressed the HEp148 protein in a partially dimerized state in HAV-susceptible cells. Immunization with the HAV-HEp148 virus induced a strong HAV- and HEV-specific immune response in mice. Thus, the present study demonstrates a novel approach to the development of a combined hepatitis A and E vaccine.
【 授权许可】
Unknown