3-Bromo-4,5-dihydroxybenzaldehyde Enhances the Level of Reduced Glutathione via the Nrf2-Mediated Pathway in Human Keratinocytes

Marine Drugs

Kyoung Ah Kang¹  Susara Ruwan Kumara Madduma Hewage¹  Mei Jing Piao¹  Hee Kyoung Kang¹  Young Sang Koh¹  Ki Cheon Kim¹  Yu Jae Hyun¹  Jin Won Hyun¹  Mee Jung Ahn² 

[1] Jeju National University School of Medicine and Jeju Research Center for Natural Medicine, Jeju 63243, Korea;Laboratory of Veterinary Anatomy, College of Veterinary Medicine, Jeju National University, Jeju 63243, Korea;

关键词: 3-bromo-4,5-dihydroxybenzaldehyde;    glutathione;    oxidative stress;    glutathione synthetase;    NF-E2 related factor 2;   

DOI  :  10.3390/md15090291

来源: DOAJ

【 摘 要 】

A natural bromophenol found in seaweeds, 3-bromo-4,5-dihydroxybenzaldehyde (BDB), has been shown to possess antioxidant effects. This study aimed to investigate the mechanism by which BDB protects skin cells subjected to oxidative stress. The effect of BDB on the protein and mRNA levels of glutathione-related enzymes and the cell survival of human keratinocytes (HaCaT cells) was investigated. BDB treatment increased the protein and mRNA levels of glutathione synthesizing enzymes and enhanced the production of reduced glutathione in HaCaT cells. Furthermore, BDB activated NF-E2-related factor 2 (Nrf2) and promoted its localization into the nucleus by phosphorylating its up-stream signaling proteins, extracellular signal–regulated kinase and protein kinase B. Thus, BDB increased the production of reduced glutathione and established cellular protection against oxidative stress via an Nrf2-mediated pathway.

【 授权许可】

Unknown