Induction of glutathione synthetase by 1,10‐phenanthroline

FEBS Letters

Sun, Yi¹ 

[1] Department of Molecular Biology, Parke-Davis Pharmaceutical Research, Division of Warner-Lambert Company, 2800 Plymouth Road, Ann Arbor, MI 48105, USA

关键词: Glutathione synthetase;    Phenanthroline;    Apoptosis;    Differential display;    p53;    OP;    1;    10-phenanthroline;    DD;    differential display;    GSH;    glutathione;    GSS;    glutathione synthetase;    RT;    reverse-transcriptase;    PCR;    polymerase chain reaction;    MnSOD;    manganese-containing superoxide dismutase;    DMSO;    dimethyl sulfoxide;   

DOI  :  10.1016/S0014-5793(97)00380-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The differential display (DD) was employed to identify the gene(s) responsible for 1,10-phenanthroline (OP)-induced apoptosis in murine tumor cells (Sun, Y., Bian, J., Wang, Y. and Jacobs, C. (1997) Oncogene 14, 385–393 [1]). An OP-inducible gene was isolated which encodes mouse glutathione synthetase (GSS). The GSS mRNA level began to increase 6 h post OP treatment and remained at a high level thereafter up to 24 h tested. Induction of GSS was found not to be associated with p53 activation. No significant induction of DNA fragmentation was detected in two murine tumor lines upon GSS transfection. This is the first observation indicating that GSS is inducible rather specifically by a metal chelator and that induction of GSS, however, is not sufficient to induce apoptosis. It may merely reflect a cellular response to OP-induced redox disturbance.

【 授权许可】

Unknown