Tools for modulating and measuring intracellular redox environment

redox environment;glutathione;porcine cells;electrochemical platform

Henderson, Jerrod A.

关键词: redox environment;    glutathione;    porcine cells;    electrochemical platform;   

Others  :  https://www.ideals.illinois.edu/bitstream/handle/2142/16070/1_Henderson_Jerrod.pdf?sequence=3&isAllowed=y

美国|英语

来源: The Illinois Digital Environment for Access to Learning and Scholarship

【 摘 要 】

Intracellular redox environment, the relative amount of oxidized and reduced chemical species within a cell, is important in regulating cellular processes; however, molecular mechanisms that mediate redox environment are poorly understood largely because of limitations of tools to both modulate and measure the intracellular redox environment. For example, pharmacological methods that modulate intracellular redox environment often lack selectivity, cause cellular toxicity, and perturb intracellular homeostasis. Methods for monitoring intracellular redox environment can also cause toxicity and protocols typically require disruption of cells. The overall goal of my dissertation research therefore was to develop and validate tools for both modulating and measuring the intracellular redox environment.This dissertation describes the development of three-electrode electrochemical platforms to modulate the intracellular redox environment of mammalian cells. The platforms are capable of both oxidizing and reducing the intracellular redox environment of Chinese hamster ovary (CHO) cells in correspondence to applied extracellular potential. I also demonstrate the utility of novel FRET-based biosensors, developed by collaborators, as real-time indicators of reduction/oxidation conditions in CHO and isogenic porcine fibroblasts. These studies will help unravel the role of redox regulation in many biological processes including those connected to normal and tumorigenic cells.