Molecular Characterization of Potato virus V (PVV) Infecting Solanum phureja Using Next-Generation Sequencing

Acta Biológica Colombiana

Mauricio Marín Montoya¹  Pablo Andrés Gutiérrez Sánchez¹  Daniela Álvarez Yepes¹ 

[1] Universidad Nacional de Colombia sede Medellín;

关键词: DAS-ELISA;    NGS;    papa;    Potyvirus;    RT-qPCR;   

DOI  :  10.15446/abc.v21n3.54712

来源: DOAJ

【 摘 要 】

Viral diseases are one of the most limiting problems in the production of potato worldwide. Solanum phureja constitutes one of the most susceptible materials to viral diseases in Colombia; however, there are few studies on viruses infecting this crop. In the current study, we performed a molecular characterization of Potato virus V (PVV) that infects S. phureja, using different potato plots located in the province of Antioquia, using Next-Generation Sequencing (NGS), DAS-ELISA, real time RT-PCR (RT-qPCR) and RT-PCR. Results revealed variable levels of incidence among plots (6.7 %-86 %) and the presence of two slightly different variants (Var_A and Var_B) present in approximately 72 %:28 % ratio. These PVV strains have a genome of 9828 nt codifying for a polyprotein of 3066 aa and share high nucleotide sequence identity (99,7 % in their complete genome) with respect to PVV-Phureja, recently described in Colombia, but are very divergent with respect to currently available PVV genomes (82-83 %). The genome information was used to design two sets of primers, useful in the specific detection of this virus in S. phureja leaf samples through RT-PCR (459 bp) and RT-qPCR (89 bp, Ct=12.08-21.86; Tm=78.7 °C-80.2 °C). This study underscores the importance of including diagnostics of PVV in S. phureja tuber-seed certification programs in Colombia.

【 授权许可】

Unknown