Multivariate analysis using high definition flow cytometry reveals distinct T cell repertoires between the foetal-maternal interface and the peripheral blood

Frontiers in Immunology

Scott eBurrows¹  Michelle eNeller¹  Rebekah eBrennan¹  John J Miles¹  Ralph eNanan²  Brigittie eSantner-Nanan²  Peter eHsu²  Steven eJoung² 

[1] QIMR Berghofer Medical Research Institute;Sydney Medical School, The University of Sydney;

关键词: Placenta;    CD4+ T cells;    CD8+ T cells;    T cell receptor (TCR);    Treg cells;    Peripheral Blood;   

DOI  :  10.3389/fimmu.2014.00033

来源: DOAJ

【 摘 要 】

The human T-cell compartment is a complex system and while some information is known on repertoire composition and dynamics in the peripheral blood, little is known on repertoire composition at different anatomical sites. Here, we determine the T-cell receptor β-variable (TRBV) repertoire at the decidua and compare it with the peripheral blood during normal pregnancy and preclampsia. We found total T-cell subset disparity of up to 58% between sites, including large signature TRBV expansions unique to the foetal-maternal interface. Defining the functional nature and specificity of compartment-specific T-cells will be necessary if we are to understand localised immunity, tolerance and pathogenesis.

【 授权许可】

Unknown