Oxidative Potential Induced by Ambient Particulate Matters with Acellular assays: A Review

Processes

Xinchun Liu¹  Shumin Zhou²  Hui Lu³  Weiqian Wang⁴  Kai Xiao⁴  Qingyue Wang⁴  Luying Zhang⁵  Xingzi Wang⁵  Lanfang Rao⁵  Tingting Xie⁵  Senlin Lu⁵ 

[1] Institute of Desert Meterorology, China Meteorological Administration, Urumqi 83002, China;Lab of Plant Cell Biology, Shanghai Key Laboratory of Bio-Energy Crops, School of Life Sciences, Shanghai University, Shanghai 200444, China;School of Environmental Sciences, Guangxi Normal University, Guilin 541004, China;School of Science and Engineering, Saitama University, Saitama 338-8570, Japan;School of environmental and chemical engineering, Shanghai University, Shanghai 200444, China;

关键词: ambient particles;    oxidative potential;    acellular assay;    transition metals;    quinones;   

DOI  :  10.3390/pr8111410

来源: DOAJ

【 摘 要 】

Acellular assays of oxidative potential (OP) induced by ambient particulate matters (PMs) are of great significance in screening for toxicity in PMs. In this review, several typical OP measurement techniques, including the respiratory tract lining fluid assay (RTLF), ascorbate depletion assay (AA), dithiothreitol assay (DTT), chemiluminescent reductive acridinium triggering (CRAT), dichlorofluorescin assay (DCFH) and electron paramagnetic/spin resonance assay (EPR/ESR) are discussed and their sensitivity to different PMs species composition, PMs size distribution and seasonality is compared. By comparison, the DTT assay tends to be the preferred method providing a more comprehensive measurement with transition metals and quinones accumulated in the fine PMs fraction. Specific transition metals (i.e., Mn, Cu, Fe) and quinones are found to contribute OP^DTT directly whereas the redox properties of PMs species may be changed by the interactions between themselves. The selection of the appropriate OP measurement methods and the accurate analysis of the relationship between the methods and PM components is conducive to epidemiological researches which are related with oxidative stress induced by PMs exposure.

【 授权许可】

Unknown