INFLUENCE OF SICKLE CELL GENE ON THE ALLELIC DIVERSITY AT THE MSP-1 LOCUS OF PLASMODIUM FALCIPARUM IN ADULT PATIENTS WITH SEVERE MALARIA

Mediterranean Journal of Hematology and Infectious Diseases

Bipin Kishore Kullu¹  Dilip Kumar Patel²  Chhatray Marndi³  Snehadhini Dehury⁴  Satyabrata Meher⁴  Kishalaya Das⁵  Siris Patel⁶  Prasanta Purohit⁷  Ranjeet Singh Mashon⁸  Padmalaya Das⁹ 

[1] Assistant Professor, Department of Medicine, Veer Surendra Sai Medical College, Burla, Sambalpur, Odisha, India.;Associate Professor, Department of Medicine, Head, Sickle Cell Clinic and Molecular Biology Laboratory, Project Coordinator, Odisha Sickle Cell Project, Veer Surendra Sai Medical College, Burla, Sambalpur, Odisha, India;Post Graduate Student of Internal Medicine, Veer Surendra Sai Medical College, Burla, Sambalpur, Odisha, India.;Research Assistant, Sickle Cell Clinic and Molecular Biology Laboratory, Veer Surendra Sai Medical College, Burla, Sambalpur, Odisha, India.;Scientific Officer, Sickle Cell Clinic and Molecular Biology Laboratory Veer Surendra Sai Medical College, Burla, Sambalpur, Odisha, India.;Senior Medical Officer, Sickle Cell Clinic and Molecular Biology Laboratory, Veer Surendra Sai Medical College, Burla, Sambalpur, Odisha, India;Senior Research FellowIndian Council of Medical Research (ICMR)V.S.S.Medical College, Burla, Odisha, India;Senior Research Fellow, Sickle Cell Clinic and Molecular Biology Laboratory Veer Surendra Sai Medical College, Burla, Sambalpur, Odisha, India;Senior Scientist, Department of Infectious Diseases, Asian Institute of Public Health, Bhubaneswar, Odisha, India;

关键词: Sickle Gene;    Malaria;    msp-1;    Plasmodium falciparum;    Severe;    Adult;   

DOI  :  10.4084/mjhid.2015.050

来源: DOAJ

【 摘 要 】

Although several studies have supported that sickle cell trait (HbAS) protects against falciparum malaria, the exact mechanism by which sickle gene confers protection is unclear. Further, there is no information on the influence of sickle gene on parasitic diversity of P. falciparum population in severe symptomatic malaria.  This study was undertaken to assess the effect of the sickle gene on the parasite densities and diversities in hospitalized adult patients with severe falciparum malaria. The study was carried out in 166 adult hospitalized subjects with severe falciparum malaria at Sickle Cell Clinic and Molecular Biology Laboratory, Veer Surendra Sai Institute of Medical Sciences and Research, Burla, Odisha, India . They were divided into three groups on the basis of hemoglobin variants HbAA (n=104), HbAS (n=30) and HbSS (n=32). The msp-1 loci was genotyped using a PCR based methodology. The parasite densities were significantly high in HbAA compared to HbAS and HbSS. The multiplicity of infection (MOI) and multiclonicity for msp-1 were significantly low in HbSS and HbAS compared to HbAA. The prevalence of K1 (p<0 .0001) and MAD20 (p=0.0003) alleles were significantly high in HbAA. The RO33 allele was detected at a higher frequency in HbSS and HbAS, compared to K1 and MAD20. Sickle gene was found to reduce both the parasite densities and diversity of P. falciparum in adults with severe malaria.

【 授权许可】

Unknown