Root Extracts of Two Cultivars of Paeonia Species: Lipid Composition and Biological Effects on Different Cell Lines: Preliminary Results

Molecules

Dario Telese¹  Elena Fini¹  Stefano Cerini¹  Gabriele Micheletti¹  Carla Boga¹  Nicola Rizzardi²  Natalia Calonghi²  Erika Faraci²  Giovanna Farruggia²  Christian Bergamini²  Lucia Romani³ 

[1] Department of Industrial Chemistry ‘Toso Montanari’, Alma Mater Studiorum Università di Bologna Viale Del Risorgimento 4, 402136 Bologna, Italy;Department of Pharmacy and Biotechnology, University of Bologna, Via Irnerio 48, 40126 Bologna, Italy;Via Riniera 2043, 40024 Castel San Pietro Terme, Italy;

关键词: Paeonia;    root extraction;    QPI;    ROS;    cancer;    mitochondria;   

DOI  :  10.3390/molecules26030655

来源: DOAJ

【 摘 要 】

The roots of two cultivars of Paeonia, namely Paeonia officinalis “Rubra Plena” and Paeonia “Pink Hawaiian Coral”, have been extracted with chloroform. The composition of the lipid fraction, analyzed by GC–MS technique, revealed the absence of paeonol and the presence of phenol, benzoic acid, fatty acid—and some sterol—derivatives. The chloroformic extracts have been tested on normal and several cancer cell lines but showed antiproliferative activity only on the ovarian carcinoma and the osteosarcoma. The biological activity of extracts was investigated mainly by confocal microscopy, flow cytometry and quantum phase imaging. The results indicated that the root extracts induced a hyperpolarization of mitochondria and an increase in reactive oxygen species levels, without inducing cell death. These effects are associated to an increased doubling time and a retarded confluence.

【 授权许可】

Unknown