Supercritical fluid extract of Lycium chinense Miller root inhibition of melanin production and its potential mechanisms of action

BMC Complementary and Alternative Medicine

Tsong-Min Chang²  Kuei-Jen Chang³  Wang-Ping Ko³  Wan-Yu Hsieh²  Tsang-Chi Tsai²  Wen-Ying Huang²  Huey-Chun Huang¹ 

[1] Department of Medical Laboratory Science and Biotechnology, China Medical University, Taichung, Taiwan;Department of Applied Cosmetology & Master Program of Cosmetic Sciences, HungKuang University, No. 1018, Sec. 6, Taiwan Boulevard, Shalu Dist, Taichung City 43302, Taiwan;O’right Plant Extract R&D Center, Taoyuan, Taiwan

关键词: ROS;    PKA;    MAPK;    melanogenesis;    Lycium chinense Miller;   

Others  :  1087488
DOI  :  10.1186/1472-6882-14-208

 received in 2013-11-13, accepted in 2014-06-23,  发布年份 2014

【 摘 要 】

Background

The mode of action of Lycium chinense Miller root extract in skin care has never been explored. In the present study, Lycium chinense Miller root was extracted by the supercritical fluid CO₂ extraction method.

Methods

In the present study, the components of the root extract were analyzed by HPLC. The effects of the extract on tyrosinase activity and melanin content were determined spectrophotometrically; the expression of melanogenesis-related proteins was determined by Western blotting; the possible signaling pathways involved in the root extract-mediated depigmentation were also investigated using specific inhibitors.

Results

The results revealed that the SFE of Lycium chinense Miller root (2.37-7.11 mg/mL) effectively suppressed intracellular tyrosinase activity and decreased the melanin content in B16F10 cells. The root extract also effectively decreased intracellular reactive oxygen species (ROS) levels. Furthermore, the root extract decreased the expression of melanocortin 1 receptor (MC1R), microphthalmia-associated transcription factor (MITF), tyrosinase and tyrosinase-related protein-1 (TRP-1) and then inhibited melanogenesis in B16F10 cells. The root extract also showed antioxidant capacities and depleted cellular ROS.

Conclusions

Our results indicate that the SFE of Lycium chinense Miller root inhibited melanogenesis in B16F10 cells by down-regulation of both mitogen-activated protein kinases (MAPK) and protein kinase A (PKA) signaling pathways or through its antioxidant properties.

【 授权许可】

   
2014 Huang et al.; licensee BioMed Central Ltd.

【 图 表 】

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