Kazinol Q from Broussonetia kazinoki Enhances Cell Death Induced by Cu(ll) through Increased Reactive Oxygen Species

Molecules

Bai-Luh Wei¹  Ying-Chieh Chen¹ 

[1] ¹Department of Life Science, National Taitung University, Taitung 950, Taiwan ²Department of Life Science, Tzu-Chi University, Hualien 970, Taiwan

关键词: kazinol Q;    copper;    DNA breakage;    ROS;   

DOI  :  10.3390/molecules16043212

来源: mdpi

【 摘 要 】

The ability of the flavan kazinol Q (KQ) to induce DNA breakage in the presence of Cu(II) was examined by agarose gel electrophoresis using supercoiled plasmid DNA. In KQ-mediated DNA breakage reaction, the involvement of reactive oxygen species (ROS), H₂O₂ and O₂ ^- was established by the inhibition of DNA breakage by catalase and revealed DNA breakage by superoxide dismutase (SOD). The cell viability of gastric carcinoma SCM-1 cells treated with various concentrations of KQ was significantly decreased by cotreatment with Cu(II). Treatment of SCM-1 cells with 300 μM Cu(II) enhanced the necrosis induced by 100 μM KQ. Treatment of SCM-1 cells with 100 mM KQ in the presence of 300 mM Cu(II) increased the generation of H₂O₂. Taken together, the above finding suggested that KQ cotreatment with Cu(II) produced increased amounts of H₂O₂, thus enhancing subsequent cell death due to necrosis.

【 授权许可】

CC BY   
This is an open access article distributed under the Creative Commons Attribution License (CC BY) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.