Case Report: Histological and Histomorphometrical Results of a 3-D Printed Biphasic Calcium Phosphate Ceramic 7 Years After Insertion in a Human Maxillary Alveolar Ridge

Frontiers in Bioengineering and Biotechnology

Alessandra Giuliani²  Ilaria De Tullio³  Giovanna Iezzi³  Mario Raspanti⁴  Adriano Piattelli⁵  Carlo Mangano⁶ 

[1] Chair of Biomaterials Engineering, Catholic University of San Antonio de Murcia (UCAM), Murcia, Spain;Department of Clinical Sciences, Polytechnic University of Marche, Ancona, Italy;Department of Medical, Oral and Biotechnological Sciences, University “G. D'Annunzio” of Chieti-Pescara, Chieti, Italy;Department of Medicine and Surgery, University of Insubria, Varese, Italy;Fondazione Villaserena per la Ricerca, Città Sant'Angelo, Italy;Independent Researcher, Gravedona, Italy;

关键词: bone substitutes;    biomaterials;    bone regeneration;    bone augmentation;    histological analysis;    micro-ct;   

DOI  :  10.3389/fbioe.2021.614325

来源: DOAJ

【 摘 要 】

Introduction: Dental implant placement can be challenging when insufficient bone volume is present and bone augmentation procedures are indicated. The purpose was to assess clinically and histologically a specimen of 30%HA-60%β-TCP BCP 3D-printed scaffold, after 7-years.Case Description: The patient underwent bone regeneration of maxillary buccal plate with 3D-printed biphasic-HA block in 2013. After 7-years, a specimen of the regenerated bone was harvested and processed to perform microCT and histomorphometrical analyses.Results: The microarchitecture study performed by microCT in the test-biopsy showed that biomaterial volume decreased more than 23% and that newly-formed bone volume represented more than 57% of the overall mineralized tissue. Comparing with unloaded controls or peri-dental bone, Test-sample appeared much more mineralized and bulky. Histological evaluation showed complete integration of the scaffold and signs of particles degradation. The percentage of bone, biomaterials and soft tissues was, respectively, 59.2, 25.6, and 15.2%. Under polarized light microscopy, the biomaterial was surrounded by lamellar bone. These results indicate that, while unloaded jaws mimicked the typical osteoporotic microarchitecture after 1-year without loading, the BCP helped to preserve a correct microarchitecture after 7-years.Conclusions: BCP 3D-printed scaffolds represent a suitable solution for bone regeneration: they can lead to straightforward and less time-consuming surgery, and to bone preservation.

【 授权许可】

Unknown