Metabolism, ATP production and biofilm generation by Staphylococcus epidermidis in either respiratory or fermentative conditions

AMB Express

Ofelia Méndez-Romero¹  Adriana Muhlia-Almazán¹  Cristina Uribe-Alvarez²  Natalia Chiquete-Félix²  Lilia Morales-García²  Ulrik Pedroza-Dávila²  Emilio Espinoza-Simón²  Salvador Uribe-Carvajal² 

[1] Centro de Investigación y Desarrollo en Alimentos (CIAD);Department of Genetics and Molecular Biology, Instituto de Fisiología Celular, Universidad Nacional Autónoma de México (UNAM);

关键词: Staphylococcus epidermidis;    Oxygen concentration;    Metabolism;    Biofilms;    Rate of oxygen consumption;    Fermentation;   

DOI  :  10.1186/s13568-020-00966-z

来源: DOAJ

【 摘 要 】

Abstract Staphylococcus epidermidis is a Gram-positive saprophytic bacterium found in the microaerobic/anaerobic layers of the skin that becomes a health hazard when it is carried across the skin through punctures or wounds. Pathogenicity is enhanced by the ability of S. epidermidis to associate into biofilms, where it avoids attacks by the host and antibiotics. To test the effect of oxygen on metabolism and biofilm generation, cells were cultured at different oxygen concentrations ([O2]). As [O2] decreased, S. epidermidis metabolism went from respiratory to fermentative. Remarkably, the rate of growth decreased at low [O2] while a high concentration of ATP ([ATP]) was kept. Under hypoxic conditions bacteria associated into biofilms. Aerobic activity sensitized the cell to hydrogen peroxide-mediated damage. In the presence of metabolic inhibitors, biofilm formation decreased. It is suggested that at low [O2] S. epidermidis limits its growth and develops the ability to form biofilms.

【 授权许可】

Unknown